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运动神经元细胞外识别的肌肉运动游泳池<em>海兔夜蛾</em

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Extracellularly Identifying Motor Neurons for a Muscle Motor Pool in Aplysia californica

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运动神经元细胞外识别的肌肉运动游泳池<em>海兔夜蛾</em

DOI:

10.3791/50189-v

•

13:37 min

•

March 25, 2013

•

Hui Lu, Jeffrey M. McManus, Hillel J. Chiel^2,3

¹Department of Biology,Case Western Reserve University , ²Department of Neurosciences,Case Western Reserve University , ³Department of Biomedical Engineering,Case Western Reserve University

Capítulos

00:05Título
01:35Electrode Preparation
02:14Ganglia and Muscle Preparation
07:42Setting up the Extracellular Glass Electrodes for Soma Recordings
08:35Setting up the Suction Electrodes for the Nerve Recordings
09:41Setting up the Force Transducer to Measure the I1/I3 Muscle Contraction
10:38Results: Extracellularly Identifying Motor Neurons for a Muscle Motor Pool in Aplysia californica
13:19Conclusion

Summary

Tadução automática

在大确定的神经元（动物<em>例如，</em>软体动物），分析细胞内的技术是通过使用电机池<sup> 1,2,3,4</sup>。最近，我们开发出一种技术，细胞外刺激和记录单个神经元<em>海兔夜蛾</em<sup></sup>。现在我们描述了使用这种技术来唯一地识别和表征运动神经元内的电机池的协议。

Tags

Keywords: Extracellular Recording Motor Neuron Identification Motor Pool Aplysia Californica Muscle Innervation Axonal Projection Soma Size Intracellular Techniques Electrophysiology

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