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Live-cell Video Microscopy af svampepatogen Fagocytose
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Live-cell Video Microscopy of Fungal Pathogen Phagocytosis

Live-cell Video Microscopy af svampepatogen Fagocytose

DOI:
10.3791/50196-v

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08:52 min

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January 09, 2013

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, , , ,
1Division of Applied Medicine,University of Aberdeen, 2Aberdeen Fungal Group,University of Aberdeen

Capítulos

  • 00:05Título
  • 01:44C. albicans Growth and Conditions
  • 02:38C. albicans Staining Using Fluorescein Isothiocyanate (FITC)
  • 03:34Preparation of the J774.1 Mouse Macrophage Cell Line
  • 04:55Live Cell Video Microscopy Phagocytosis Assay 28
  • 06:40Analyzing the Live Cell Video
  • 08:10Conclusion

Summary

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Vi beskriver metoder til live-cell video mikroskopi af<em> Candida albicans</em> Fagocytose med makrofager. Disse metoder sikrer fase-specifik analyse af makrofag migration, anerkendelse, engulfment og phagosome modning og afslører hidtil ukendte sider af fagocytose.

Tags

Keywords: Live-cell Video MicroscopyFungal Pathogen PhagocytosisPhagocytic ClearancePattern Recognition ReceptorsPhagosome MaturationInnate Immune Cell FunctionFungal PathogenesisMacrophage UptakeCell CycleVesicular Trafficking

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