Измерение Катионит транспорта по Na, K и H, K-АТФазы<em> Xenopus</em> Ооцитов методом атомно-абсорбционной спектрофотометрии: Альтернатива радиоизотопных Анализы
We describe a method to quantify the activity of K+-countertransporting P-type ATPases by heterologous expression of the enzymes in Xenopus oocytes and measuring Rb+ or Li+ uptake into individual cells by atomic absorption spectrophotometry. The method is a sensitive and safe alternative to radioisotope flux experiments facilitating complex kinetic studies.