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Mitochondria-associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs): Isolation from Mouse Brain
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Neurociência
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JoVE Journal Neurociência
Mitochondria-associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs): Isolation from Mouse Brain

Mitochondria-associated ER Membranes (MAMs) and Glycosphingolipid Enriched Microdomains (GEMs): Isolation from Mouse Brain

DOI:
10.3791/50215-v

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10:32 min

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March 04, 2013

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, ,
1Department of Genetics,St Jude Children’s Research Hospital

Capítulos

  • 00:05Título
  • 02:21Subcellualar Fractionation: Removal of Nuclei, Unlysed Cells, and Cellular Debris
  • 04:08Crude Mitochondria Isolation
  • 06:16Isolation of Mitochondria-associated ER Membrane, MAMs
  • 08:27Extraction of Glycosphingolipid-enriched Microdomains, GEMs
  • 09:14Results: Western Blot Analysis of GEMs and MAMs
  • 10:01Conclusion

Summary

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This procedure illustrates how to isolate from the adult mouse brain the mitochondria-associated ER membranes or MAMs and the glycosphingolipid-enriched microdomain fractions from MAMs and mitochondrial preparations.

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Keywords: Mitochondria-associated ER Membranes (MAMs)Glycosphingolipid Enriched Microdomains (GEMs)Intracellular OrganellesEndoplasmic Reticulum (ER)MitochondriaCa2+ SignalingLipid BiosynthesisEnergy MetabolismCell SurvivalMembrane Contact SitesTriton X100 ExtractionCaveolaeLipid Rafts

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