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Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples
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JoVE Journal Química
Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

Rapid Colorimetric Assays to Qualitatively Distinguish RNA and DNA in Biomolecular Samples

DOI:
10.3791/50225-v

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05:52 min

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February 04, 2013

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,
1Department of Chemistry,University of Virginia

Capítulos

  • 00:05Título
  • 01:33Benedict’s Assay
  • 02:50Bial’s Orcinol Assay
  • 03:45Dische’s Diphenylamine Assay
  • 04:26Representative Assay Results
  • 05:36Conclusion

Summary

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Tadução automática

A suite of colorimetric assays is described for rapidly distinguishing protein, RNA, DNA, and reducing sugars in potentially heterogeneous biomolecular samples.

Tags

Colorimetric AssayRNADNABiomolecular SampleSpectrophotometricFluorometricNucleic AcidProteinBenedict’s ReagentBial’s ReagentDische’s ReagentPentoseCarbohydrateReducing Sugar

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