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Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research
JoVE Journal
Medicina
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JoVE Journal Medicina
Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research

DOI:
10.3791/50238-v

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15:18 min

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January 12, 2013

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, , , , , , , , , , ,
1Umeå Centre for Molecular Medicine,Umeå University, 2Cell Transplant Center, Diabetes Research Institute,University of Miami,, 3EMBL-CRG Systems Biology Program, Centre for Genomic Regulation,Catalan Institute of Research and Advanced Studies, 4Dept. of Computing Science,Umeå University

Capítulos

  • 00:00Título
  • 01:18Introduction
  • 02:02The Near Infrared Optical Projection Tomography (NIR-OPT) Setup
  • 03:31Sample Preparation and Scanning
  • 10:31Computational Processing and Reconstruction
  • 12:38Representative Results
  • 14:46Conclusions

Summary

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We describe the adaptation of optical projection tomography (OPT)1 to imaging in the near infrared spectrum, and the implementation of a number of computational tools. These protocols enable assessments of pancreatic β-cell mass (BCM) in larger specimens, increase the multichannel capacity of the technique and increase the quality of OPT data.

Tags

Near Infrared Optical Projection TomographyBeta-cell Mass DistributionDiabetes ResearchMulti-channel ImagingComputational ToolsCenter Of Mass PositioningPost-alignment TuningIntensity EqualizationSample HolderIslet Transplantation

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