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近红外光学投影层析成像技术在糖尿病研究β细胞质量分布的评估
JoVE Journal
Medicina
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JoVE Journal Medicina
Near Infrared Optical Projection Tomography for Assessments of β-cell Mass Distribution in Diabetes Research
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近红外光学投影层析成像技术在糖尿病研究β细胞质量分布的评估

DOI:
10.3791/50238-v

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15:18 min

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January 12, 2013

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, , , , , , , , , , ,
1Umeå Centre for Molecular Medicine,Umeå University, 2Cell Transplant Center, Diabetes Research Institute,University of Miami,, 3EMBL-CRG Systems Biology Program, Centre for Genomic Regulation,Catalan Institute of Research and Advanced Studies, 4Dept. of Computing Science,Umeå University

Capítulos

  • 00:00Título
  • 01:18Introduction
  • 02:02The Near Infrared Optical Projection Tomography (NIR-OPT) Setup
  • 03:31Sample Preparation and Scanning
  • 10:31Computational Processing and Reconstruction
  • 12:38Representative Results
  • 14:46Conclusions

Summary

Tadução automática

Tadução automática

我们描述了适应光学投影层析成像（OPT）<sup> 1</sup>成像技术在近红外光谱，并实施了一些计算工具。这些协议使胰岛β-细胞团（BCM）的评估，在更大的标本，增加多渠道的技术能力，并增加OPT数据的质量。

Tags

Near Infrared Optical Projection TomographyBeta-cell Mass DistributionDiabetes ResearchMulti-channel ImagingComputational ToolsCenter Of Mass PositioningPost-alignment TuningIntensity EqualizationSample HolderIslet Transplantation

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