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活细胞周期分析<em>果蝇</em>组织使用同调声聚焦流式细胞仪Vybrant的DyeCycle紫DNA染色
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Biologia
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JoVE Journal Biologia
Live Cell Cycle Analysis of Drosophila Tissues using the Attune Acoustic Focusing Cytometer and Vybrant DyeCycle Violet DNA Stain
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活细胞周期分析<em>果蝇</em>组织使用同调声聚焦流式细胞仪Vybrant的DyeCycle紫DNA染色

DOI:
10.3791/50239-v

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11:00 min

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May 19, 2013

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, , , ,
1Molecular, Cellular and Developmental Biology,University of Michigan

Capítulos

  • 00:05Título
  • 01:44Fly Husbandry and Dissection
  • 04:04Tissue Dissociation and DNA Staining
  • 05:16Flow Cytometry
  • 07:52Results: Live Cell Cycle Analysis
  • 10:41Conclusion

Summary

Tadução automática

Tadução automática

活细胞周期分析的一种协议<em>果蝇</em>组织使用同调声对焦流式细胞仪进行说明。该协议同时通过提供信息相对细胞大小，细胞数量，DNA含量及细胞类型谱系跟踪或组织特异性表达的荧光蛋白<em>在体内</em>。

Tags

Cell Cycle AnalysisDrosophilaFlow CytometryAttune Acoustic Focusing CytometerVybrant DyeCycle VioletDNA StainLive-cell AnalysisCell-type Specific AnalysisCell SizeCell Number

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