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Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

Monitoring Dendritic Cell Migration using 19F / 1H Magnetic Resonance Imaging

DOI:
10.3791/50251-v

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08:12 min

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March 20, 2013

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, , , , , ,
1Experimental and Clinical Research Center,A joint cooperation between the Charité Medical Faculty and the Max Delbrück Center for Molecular Medicine, 2Berlin Ultrahigh Field Facility (B.U.F.F.),Max Delbrück Center for Molecular Medicine

Capítulos

  • 00:05Título
  • 01:46Labeling and In vivo Application of Dendritic Cells (DC) with 19F (Fluorine)-rich Particles
  • 02:47In vivo 19F/1H (Proton) Magnetic Resonance Imaging (MRI)
  • 05:04Lymph Node Magnetic Resonance Spectroscopy (MRS)
  • 06:20Results: Quantification of 19F-labeled DC Migration In vivo using 19F MRS
  • 07:33Conclusion

Summary

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Tadução automática

Tracking of cells using MRI has gained remarkable attention in the past years. This protocol describes the labeling of dendritic cells with fluorine (19F)-rich particles, the in vivo application of these cells, and monitoring the extent of their migration to the draining lymph node with 19F/1H MRI and 19F MRS.

Tags

Magnetic Resonance ImagingMRICell TrackingDendritic CellsFluorine-19PerfluorocarbonCell LabelingCell MigrationIn Vivo ImagingContrast AgentsUSPIOT2 EnhancementBackground-free ImagingCell QuantificationMR Spectroscopy

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