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液泡和胞质pH值的测量<em>在体内</em在酵母细胞悬浮液
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Biologia
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JoVE Journal Biologia
Measurement of Vacuolar and Cytosolic pH In Vivo in Yeast Cell Suspensions
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液泡和胞质pH值的测量<em>在体内</em在酵母细胞悬浮液

DOI:
10.3791/50261-v

•

13:55 min

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April 19, 2013

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, ,
1Department of Biochemistry and Molecular Biology,SUNY Upstate Medical University

Capítulos

  • 00:05Título
  • 02:12Measurement of Vacuolar pH In Vivo using BCECF-AM
  • 07:17Measurement of Cytosolic pH In Vivo using Yeast pHluorin
  • 11:54Results: Vacuolar and Cytosolic pH Changes in Response to Glucose and Other Stimuli
  • 13:25Conclusion

Summary

Tadução automática

Tadução automática

可以测量活酵母液泡膜和细胞内pH值（<em> S。酵母</em>）定位到特定的细胞区室的使用比例荧光染料的细胞。我们描述BCECF-AM，定位于液泡酵母，细胞内pH值与胞浆的比例的pH敏感GFP（酵母pHluorin所指示）测量空泡pH值的程序。

Tags

Vacuolar PHCytosolic PHPH HomeostasisYeastBCECFYeast PHluorinRatiometric MeasurementFluorescenceFluorimeterFluorescence Plate Reader

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