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Capítulos
- 00:01Título
- 01:12Design and Use of Multiplexed Chemostat Arrays
- 01:18How the Ministats Work
- 02:03Anatomy of the Array
- 03:11How to Clean and Assemble a Ministat Array
- 03:40Marking Culture Tubes at the Desired Working Culture Volume
- 04:11Making the Cork Assembly
- 05:48Setting Up the Gas Washing Bottle to Hydrate Air and Prevent Evaporation
- 06:39Use of a 4-Port Manifold to Divide Air Flow Between Multiple Chambers
- 07:35Setting the Peristaltic Pump and Heating Block
- 08:24Air Tubing Assembly
- 08:50Effluent Tube Assembly
- 09:08Media Tubing Assembly
- 10:21Preparing Effluent Sampling Containers
- 11:01Part Assembly and Autoclaving
- 11:34Connecting all Tubes to the Culture Chamber
- 12:33Preparing the Carboy and Autoclaving
- 12:55Running Experiments
- 15:13Time Zero Sampling
- 16:08Daily Measurements
- 16:52Taking Down an Experiment and Cleaning Up
Vi udviklet og valideret en lille fodaftryk vifte af miniature kemostater bygget af let tilgængelige dele til en lav pris. Fysiologiske og eksperimentel udvikling skyldes svarede til større volumen kemostater. Den ministat arrayet giver en kompakt, billig og tilgængelig platform for traditionelle kemostat eksperimenter, funktionelle genomics og kemiske screening applikationer.
