Journal
/
Biologia
/
Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells

Generation of Dispersed Presomitic Mesoderm Cell Cultures for Imaging of the Zebrafish Segmentation Clock in Single Cells

DOI:
10.3791/50307-v

•

10:41 min

•

July 24, 2014

•

, , , ,
1Max Planck Institute of Molecular Cell Biology and Genetics

Capítulos

  • 00:05Título
  • 01:39Dissection Preparation
  • 03:15Presomitic Mesoderm Dissection and Dispersal
  • 06:40Imaging Dispersed Presomitic Mesodermal Cells
  • 07:58Image Processing of Acquired Time-lapse Movies
  • 08:58Representative Time-lapse Imaging Results
  • 10:00Conclusion

Summary

Tadução automática

Tadução automática

Somitogenesis is a rhythmic developmental process that spatially patterns the body axis of vertebrate embryos. Previously, we developed transgenic zebrafish lines that use fluorescent reporters to observe the cyclic genes that drive this process. Here, we culture dispersed cells from these lines and image their oscillations over time in vitro.

Tags

Keywords: ZebrafishSegmentationPresomitic MesodermSomitesSegmentation ClockHer1Fluorescent ReporterIn Vitro CultureSingle-cell ImagingTime-lapse Imaging

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/50307?article_type=v

Single Cell Fate Mapping in Zebrafish
check_url/pt/50307?article_type=v

In vivo Electroporation of Morpholinos into the Adult Zebrafish Retina
check_url/pt/50307?article_type=v

In vivo Electroporation of Morpholinos into the Regenerating Adult Zebrafish Tail Fin
check_url/pt/50307?article_type=v

Generation of Mice Derived from Induced Pluripotent Stem Cells
check_url/pt/50307?article_type=v

Monitoring Cell-autonomous Circadian Clock Rhythms of Gene Expression Using Luciferase Bioluminescence Reporters
check_url/pt/50307?article_type=v

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis
check_url/pt/50307?article_type=v

Efficient Generation Human Induced Pluripotent Stem Cells from Human Somatic Cells with Sendai-virus
check_url/pt/50307?article_type=v

Nephrotoxin Microinjection in Zebrafish to Model Acute Kidney Injury
check_url/pt/50307?article_type=v

Analysis of Beta-cell Function Using Single-cell Resolution Calcium Imaging in Zebrafish Islets
check_url/pt/50307?article_type=v

Probing mRNA Kinetics in Space and Time in Escherichia coli using Two-Color Single-Molecule Fluorescence In Situ Hybridization

Read Article