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直接成像诱导ER钙有针对性的酯酶染料装载（TED）

JoVE Journal
Biologia

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JoVE Journal Biologia

Direct Imaging of ER Calcium with Targeted-Esterase Induced Dye Loading (TED)

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直接成像诱导ER钙有针对性的酯酶染料装载（TED）

DOI:

10.3791/50317-v

•

09:32 min

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May 07, 2013

•

Samira Samtleben, Juliane Jaepel², Caroline Fecher, Thomas Andreska, Markus Rehberg, Robert Blum

¹Institute for Clinical Neurobiology,University of Wuerzburg, ²Department of Synapses – Circuits – Plasticity,Max Planck Institute of Neurobiology, Martinsried, ³Walter Brendel Centre of Experimental Medicine,Ludwig-Maximilians University of Munich

Capítulos

00:05Título
02:07Preparation of Live Cell Imaging
02:50Dye Loading of Cells
04:06ER-calcium Live Cell Imaging with an Inverted Laser Scanning Confocal Microscope
05:57Image Processing and Data Analysis
06:38Representative Endoplasmic Reticulum Calcium Imaging Results
08:18Conclusion

Summary

Tadução automática

有针对性的酯酶诱导染料吸附量（TED）支持通过荧光成像分析细胞内钙库动态。该方法的基础上针对重组羧酸酯酶的内质网（ER），在那里它提高了合成的低亲和性钙本地揭露<sup> +</sup>指示剂染料在ER腔。

Tags

Calcium Dynamics ER Calcium TED Targeted-esterase Induced Dye Loading Calcium Indicators Fluo5N Mag-Fluo4 Mag-Fura2 Carboxylesterase Lentivirus Live Cell Imaging ER Structure Calcium Release Calcium Refilling

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