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开发，扩展，<em>在体内</em>从人类胚胎干细胞（hESCs）监测人类NK细胞和诱导多能干细胞（iPS细胞）
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JoVE Journal Bioengenharia
Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)
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开发，扩展，<em>在体内</em>从人类胚胎干细胞（hESCs）监测人类NK细胞和诱导多能干细胞（iPS细胞）

DOI:
10.3791/50337-v

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09:02 min

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April 23, 2013

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, ,
1Department of Medicine (Hematology, Oncology, and Transplant),University of Minnesota, Minneapolis, 2Stem Cell Institute,University of Minnesota, Minneapolis

Capítulos

  • 00:05Título
  • 01:18Setting up TrypLE-passaged ES Cells into Stage I Spin Embryoid Bodies (EBs): Day of Spin EB Setup into Stage I Differentiation (Day 0)
  • 03:16Development, Expansion, and Characterization of NK Cells from hPSC-derived Progenitor Cells
  • 04:19In vivo Fluorescent and Bioluminescent Imaging to Monitor hESC-derived NK Cells and Tumor Cells in Immunodeficient Mice
  • 06:50Results: The Generation of Hematopoietic Progenitor Cells Using the Spin EB Approach Allows for Optimal NK Cell Development from hESCs and iPSCs
  • 08:43Conclusion

Summary

Tadução automática

Tadução automática

本协议描述的发展，扩张和来自人类胚胎干细胞和iPS细胞的NK细胞在体内成像。

Tags

Human Embryonic Stem CellsInduced Pluripotent Stem CellsNatural Killer CellsFeeder-free DifferentiationArtificial Antigen Presenting CellsCell ExpansionMature PhenotypeGenetic ModificationFirefly LuciferaseIn Vivo MonitoringDual-imagingAdoptive Cell Therapy

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