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פיתוח, הרחבה, ו<em> בvivo</emתאי גזע pluripotent> ניטור של תאי NK אדם מתאי גזע עובריים אנושיים (hESCs) ומושרה (iPSCs)
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Development, Expansion, and In vivo Monitoring of Human NK Cells from Human Embryonic Stem Cells (hESCs) and Induced Pluripotent Stem Cells (iPSCs)
DOI:

09:02 min

April 23, 2013

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Capítulos

  • 00:05Título
  • 01:18Setting up TrypLE-passaged ES Cells into Stage I Spin Embryoid Bodies (EBs): Day of Spin EB Setup into Stage I Differentiation (Day 0)
  • 03:16Development, Expansion, and Characterization of NK Cells from hPSC-derived Progenitor Cells
  • 04:19In vivo Fluorescent and Bioluminescent Imaging to Monitor hESC-derived NK Cells and Tumor Cells in Immunodeficient Mice
  • 06:50Results: The Generation of Hematopoietic Progenitor Cells Using the Spin EB Approach Allows for Optimal NK Cell Development from hESCs and iPSCs
  • 08:43Conclusion

Summary

Tadução automática

פרוטוקול זה מתאר את הפיתוח, הרחבה, ובתחום ההדמיה vivo של תאי NK הנגזרים מhESCs וiPSCs.

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