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评估神经退行性表型<em>果蝇</em>爬坡试验和全脑多巴胺能神经元免疫组化染色
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Neurociência
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JoVE Journal Neurociência
Assessing Neurodegenerative Phenotypes in Drosophila Dopaminergic Neurons by Climbing Assays and Whole Brain Immunostaining
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评估神经退行性表型<em>果蝇</em>爬坡试验和全脑多巴胺能神经元免疫组化染色

DOI:
10.3791/50339-v

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09:17 min

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April 24, 2013

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1Department of Biomolecular Genetics,University of Rochester Medical Center

Capítulos

  • 00:05Título
  • 01:10Startle-induced Negative Geotaxis Assay
  • 03:14Negative Geotaxis Assay Data Analysis
  • 04:07Dissecting Adult Drosophila Brains for Whole Mounts
  • 05:14Tyrosine Hydroxylase Immunofluorescence of Whole Mount Brains
  • 08:10Investigating the Stress-protective Nrf2 Pathway in a Drosophila Model of PD
  • 08:56Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们描述了两个实验，已建立了学习年龄依赖性的多巴胺（DA）神经元的神经退行性疾病<em>果蝇</em>：攀登/的惊跳诱导负趋地性试验，允许DA能神经元变性，这是用来识别和计数DA能神经元全脑坐骑酪氨酸羟化酶免疫功能的影响研究。

Tags

DrosophilaDopaminergic NeuronsNeurodegenerative PhenotypesClimbing AssayWhole Brain ImmunostainingParkinson’s DiseaseAlpha-synucleinTyrosine Hydroxylase

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