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Recombineering homolog rekombination konstruktioner i<em> Drosophila</em
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Biologia
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JoVE Journal Biologia
Recombineering Homologous Recombination Constructs in Drosophila

Recombineering homolog rekombination konstruktioner i<em> Drosophila</em

DOI:
10.3791/50346-v

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14:23 min

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July 13, 2013

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, , , , ,
1Department of Molecular Biology,University of Texas Southwestern Medical Center at Dallas, 2Department of Physiology,University of Texas Southwestern Medical Center at Dallas, 3Green Center for Systems Biology,University of Texas Southwestern Medical Center at Dallas

Capítulos

  • 00:05Título
  • 01:53Selection of BAC Clone and Region to Target
  • 03:04Insert the Homology Arms into P[acman]-KO 1.0
  • 04:40Recombining the Genomic Region of Interest into P[acman]-KO 1.0
  • 08:59Replacing the Genomic Region with the Targeting Cassette
  • 12:30Anticipated Yields
  • 13:42Conclusion

Summary

Tadução automática

Tadução automática

Homolog rekombinationsteknikker høj grad fremme<em> Drosophila</em> Genetik ved at muliggøre skabelsen af ​​molekylært præcise mutationer. Den nylige vedtagelse af recombineering tillader en at manipulere store stykker af DNA og omdanne dem til<em> Drosophila</em<sup> 6</sup>. De metoder, der præsenteres her kombinere disse teknikker til hurtigt at frembringe store homologe rekombinationsvektorer.

Tags

Keywords: RecombineeringHomologous RecombinationDrosophilaBAC TransgenesisEnds-out Homologous RecombinationGateway TechnologyGene TargetingKnockoutKnock-inGenomic Manipulation

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