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核小体阵列从重组核心组蛋白和核小体定位的DNA组装
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Biologia
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JoVE Journal Biologia
Assembly of Nucleosomal Arrays from Recombinant Core Histones and Nucleosome Positioning DNA
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核小体阵列从重组核心组蛋白和核小体定位的DNA组装

DOI:
10.3791/50354-v

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10:40 min

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September 10, 2013

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, , , , ,
1Biochemistry and Molecular Biology,Colorado State University

Capítulos

  • 00:05Título
  • 01:37Assembly of Recombinant Core Histones into Octamers
  • 03:14Purification of Histone Octamers by Size Exclusion Chromatography |
  • 05:32Sedimentation Velocity Analysis of Reconstituted Nucleosomal Arrays
  • 07:50Results: Nucleosomal Arrays from Recombinant Xenopus Core Histones
  • 09:56Conclusion

Summary

Tadução automática

Tadução automática

的方法，提出了用于从重组核心组蛋白模型核小体阵列的重构和串联重复的核小体定位的DNA。我们还描述沉降速度实验在分析型超速离心机，和原子力显微镜（AFM）是如何被用来监测核小体阵列饱和的重构后的程度。

Tags

Nucleosomal ArrayRecombinant Core HistonesNucleosome Positioning DNASedimentation VelocityAnalytical UltracentrifugeAtomic Force MicroscopyChromatin StructureChromatin Function

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