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肽为基础的识别功能图案和具有约束力的合作伙伴
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Peptide-based Identification of Functional Motifs and their Binding Partners
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肽为基础的识别功能图案和具有约束力的合作伙伴

DOI:
10.3791/50362-v

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14:28 min

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June 30, 2013

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, , , , , ,
1Department of Microbiology, Biochemistry, & Immunology,Morehouse School of Medicine, 2Institute for Systems Biology, 3Advanced Medical & Dental Institute,Universiti Sains Malaysia

Capítulos

  • 00:05Título
  • 02:55Nef Scanning Peptide TUNEL Assay
  • 04:43Competitive Inhibition Analysis Using Peptides
  • 06:02Isolating Motif Binding Partners Using Peptides as Bait
  • 09:25Nef-GFP Secretion Fluorescent Plate Reader Assay of Antibody Inhibition
  • 10:33Identification of Nef Functional Motifs and their Binding Partners
  • 13:23Conclusion

Summary

Tadução automática

Tadução automática

解剖机制的分泌HIV-1 Nef的外来技术进行了阐述。来自NEF和蛋白转染的特殊短肽被利用来确定Nef的分泌修改区域的结构，功能和有约束力的合作伙伴。这些程序在许多机理研究具有普遍意义。

Tags

PeptideMotifBinding PartnerHIV-1 NefApoptosisSecretion Modification Region (SMR)ExosomeMortalinInhibitor

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