É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:09Material Preparation
- 02:24Seed Dissection and Embryo Extraction
- 03:49Embryo Isolation
- 06:23Results: Isolated Plant Embryos Are Suitable for Diverse Downstream Applications
- 07:36Conclusion
We report an efficient and simple method to isolate embryos at early stages of development from Arabidopsis thaliana seeds. Up to 40 embryos can be isolated in 1 hr to 4 hr, depending on the downstream application. The procedure is suitable for transcriptome, DNA methylation, reporter gene expression, immunostaining and fluorescence in situ hybridization analyses.
