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多光谱定量分析继荧光组织移植的细胞起源，类型和相互作用的可视化

JoVE Journal
Medicina

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JoVE Journal Medicina

Quantitative Multispectral Analysis Following Fluorescent Tissue Transplant for Visualization of Cell Origins, Types, and Interactions

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多光谱定量分析继荧光组织移植的细胞起源，类型和相互作用的可视化

DOI:

10.3791/50385-v

•

11:27 min

•

September 22, 2013

•

Erika L. Spaeth, Christopher M. Booth, Frank C. Marini²

¹Department of Leukemia,MD Anderson Cancer Center, ²Wake Forest Baptist Medical Center,Institute for Regenerative Medicine

Capítulos

00:05Título
02:06Syngeneic Murine Bone Marrow Transplantation (BMT)
03:11Tumor Engraftment
04:01Multi-parameter Immunofluorescent Staining
06:13Multispectral Imaging
07:10Quantitative Analysis of Multispectral Images
08:00Resultados
10:32Conclusion

Summary

Tadução automática

复合组织群众，从机关到肿瘤，是由各种细胞成分的。我们阐明了利用多标记的荧光转基因小鼠与多参数免疫荧光染色结合组织随后光谱分离破译细胞起源以及基于蛋白表达细胞特征的细胞内表型的贡献。

Tags

Multispectral Analysis Cell Origins Cell Types Cell Interactions Tumor Development Bone Marrow Tumor Stroma GFP RFP MIMicc Multiplexed Cellular Compositions Fluoroprobes Immunohistochemistry Cell Lineages Cell Differentiation Tumor Stromal Populations

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