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遗传操纵鼠标的发展下丘脑通过<em>在子宫内</em>电穿孔

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Genetic Manipulation of the Mouse Developing Hypothalamus through In utero Electroporation

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遗传操纵鼠标的发展下丘脑通过<em>在子宫内</em>电穿孔

DOI:

10.3791/50412-v

•

11:48 min

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July 24, 2013

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Roberta Haddad-Tóvolli*¹, Nora-Emöke Szabó*², Xunlei Zhou, Gonzalo Alvarez-Bolado

¹Institute of Anatomy and Cell Biology,University of Heidelberg , ²Institut de recherches cliniques de Montreal

Capítulos

00:05Título
02:06Preparation of DNA and Glass Micropipettes for Injection
02:57Laparotomy
04:33DNA Injection into the Brain Ventricle
05:59Targeting the Hypothalamus for Electroporation
07:17Finishing the Surgery
08:28Analyzing the Results
09:18Results: Hypothalamus Transfection by In utero Electroporation
10:54Conclusion

Summary

Tadução automática

尽管下丘脑的功能和医学重要性，<em>在子宫内</em>遗传操纵其发展已经很少有人尝试。我们显示的详细步骤<em>在子宫内</em>电到老鼠的下丘脑和展示具有代表性的结果整体和局部（区域）下丘脑转。

Tags

Keywords: Genetic Manipulation Mouse Developing Hypothalamus In Utero Electroporation Genetic Modification Mammalian Brain Mouse Mutants Developing Brain Cortex Thalamus Hypothalamus Hypothalamic Neuroepithelium Mammillary Body Electroporation

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