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耦合检测监控蛋白复性<em>酿酒酵母（Saccharomyces cerevisiae）</em
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Biologia
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JoVE Journal Biologia
Coupled Assays for Monitoring Protein Refolding in Saccharomyces cerevisiae
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耦合检测监控蛋白复性<em>酿酒酵母（Saccharomyces cerevisiae）</em

DOI:
10.3791/50432-v

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13:52 min

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July 09, 2013

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1Department of Microbiology and Molecular Genetics, Graduate School of Biomedical Sciences,University of Texas Medical School

Capítulos

  • 00:05Título
  • 01:46Construction of Strains Containing FFL-GFP Plasmid
  • 02:23Preparations Prior to FFL-GFP Induction and Enzymatic Recovery Assay
  • 03:48Induction of FFL-GFP
  • 06:22Enzymatic FFL-GFP Recovery Assay
  • 07:35Fluorescence Microscopy
  • 08:55Single Cell Microscopy
  • 10:47Results: Hsp104 is Required for Efficient Refolding of Heat-denatured FFL-GFP
  • 13:36Conclusion

Summary

Tadução automática

Tadução automática

本文介绍了利用萤火虫荧光素-GFP融合蛋白进行调查<em>在地体内</em>蛋白质折叠<em>酿酒酵母（Saccharomyces cerevisiae）</em>。使用这种试剂，可同时监控一个模型的热变性蛋白复性，通过荧光显微镜和酶法测定蛋白质的质量控制中，以探测作用的proteostasis网络组件。

Tags

Keywords: Protein RefoldingProteostasisProtein FoldingProtein AggregationFirefly LuciferaseGreen Fluorescent ProteinFFL-GFPHsp104Hsp40Hsp70Nucleotide Exchange FactorProtein ChaperonesProtein Quality ControlNeurodegenerative Disorders

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