Journal
/
Biologia
/
Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

Production of Xenopus tropicalis Egg Extracts to Identify Microtubule-associated RNAs

DOI:
10.3791/50434-v

•

10:01 min

•

June 27, 2013

•

,
1Department of Molecular Biology,Massachusetts General Hospital, 2Department of Genetics,Harvard Medical School

Capítulos

  • 00:05Título
  • 01:15Generation of X. tropicalis Eggs
  • 03:24Preparation of Extract from X. tropicalis Eggs
  • 06:45Purification of Taxol-stabilized Microtubules from X. tropicalis Extract
  • 08:25Representative X. Tropicalis Extraction Results
  • 09:37Conclusion

Summary

Tadução automática

Tadução automática

We describe the collection of unfertilized Xenopus tropicalis eggs and production of a meiosis II-arrested egg extract. This egg extract can be used to purify microtubules and microtubule-associated RNAs.

Tags

Xenopus TropicalisEgg ExtractsMicrotubule-associated RNAsTranscript IdentificationHigh-throughput SequencingSubcellular StructuresRNA LocalizationXenopus Laevis

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Preparation and Fractionation of Xenopus laevis Egg Extracts

In Vitro Nuclear Assembly Using Fractionated Xenopus Egg Extracts

Microinjection of Xenopus Laevis Oocytes

Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation

Using SCOPE to Identify Potential Regulatory Motifs in Coregulated Genes

Study of the DNA Damage Checkpoint using Xenopus Egg Extracts

Reconstitution Of β-catenin Degradation In Xenopus Egg Extract

Isolation of Small Noncoding RNAs from Human Serum

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Xenopus laevis as a Model to Identify Translation Impairment

Read Article