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Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies
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Biologia
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JoVE Journal Biologia
Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies

Reconstitution of a Kv Channel into Lipid Membranes for Structural and Functional Studies

DOI:
10.3791/50436-v

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10:22 min

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July 13, 2013

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, , ,
1Department of Cell Biology,University of Texas Southwestern Medical Center at Dallas

Capítulos

  • 00:05Título
  • 01:27Liposome Preparation and Detergent-induced Fusion of Vesicles
  • 04:15Removal of the Detergents to Form Proteoliposomes
  • 06:28Functional Study of the Ion Channel Activities in Black Lipid Membranes
  • 08:49Results: Two-Dimensional Crystallization of KvAP in Membranes
  • 09:56Conclusion

Summary

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Procedures for complete reconstitution of a prototype voltage-gated potassium channel into lipid membranes are described. The reconstituted channels are suitable for biochemical assays, electrical recordings, ligand screening and electron crystallographic studies. These methods may have general applications to the structural and functional studies of other membrane proteins.

Tags

Keywords: Kv ChannelLipid ReconstitutionMembrane ProteinsVoltage-gated Potassium ChannelLipid-protein InteractionMembrane SystemsDetergent-induced Fusion2D Crystals

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