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RNAi介导的双基因敲除和味觉感知测量蜜蜂（<em>蜜蜂</em>）
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Neurociência
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JoVE Journal Neurociência
RNAi-mediated Double Gene Knockdown and Gustatory Perception Measurement in Honey Bees (Apis mellifera)
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RNAi介导的双基因敲除和味觉感知测量蜜蜂（<em>蜜蜂</em>）

DOI:
10.3791/50446-v

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10:57 min

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July 25, 2013

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, ,
1School of Life Sciences,Arizona State University , 2Department of Chemistry, Biotechnology, and Food Science,Norwegian University of Life Sciences

Capítulos

  • 00:05Título
  • 02:40RNAi-mediated Double Gene Knockdown
  • 05:54Proboscis Extension Response (PER) Assay
  • 07:36Results: Double Kockdown of Vitellogenin (vg) and Ultraspiracle (usp) Genes affects Gustatory Perception
  • 09:51Conclusion

Summary

Tadução automática

Tadução automática

在这个协议中，我们描述了两种策略，同时抑制这两个基因在蜜蜂（双基因敲除）。然后，我们提出了如何用长鼻延伸反应（PER）的检测，以研究双基因敲除对蜜蜂的味觉感知的效果。

Tags

RNAiDouble Gene KnockdownGustatory PerceptionProboscis Extension Response (PER) AssayHoney BeesApis MelliferaVitellogenin (vg)Ultraspiracle (usp)Gene ExpressionBehavioral MaturationForagingLearning And Memory

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