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Prostaglandin Extraction and Analysis in Caenorhabditis elegans

JoVE Journal
Biologia

This content is Free Access.

JoVE Journal Biologia

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

Prostaglandin Extraction and Analysis in Caenorhabditis elegans

DOI:

10.3791/50447-v

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08:50 min

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June 25, 2013

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Jeevan K. Prasain, Hieu D. Hoang, Johnathan W. Edmonds, Michael A. Miller

¹Department of Pharmacology and Toxicology,University of Alabama at Birmingham, ²Department of Cell, Developmental, and Integrative Biology,University of Alabama at Birmingham

Capítulos

00:05Título
01:05Worm Culture
03:28Prostaglandin Extraction
06:14Prostaglandin Analysis
07:15Results: Prostaglandin Extraction and Analysis in C. elegans
08:22Conclusion

Summary

Tadução automática

In this paper, we describe an optimized procedure for extracting and analyzing prostaglandins and other eicosanoids from C. elegans using LC-MS/MS.

Tags

Keywords: Prostaglandin Caenorhabditis Elegans Eicosanoid Lipid Extraction Liquid Chromatography-mass Spectrometry Tandem Mass Spectrometry Multiple Reaction Monitoring Collision-induced Decomposition

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