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Imunologia e Infecção

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4D多模态成像<em>柠檬酸rodentium</em在小鼠感染

JoVE Journal
Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

4D Multimodality Imaging of Citrobacter rodentium Infections in Mice

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4D多模态成像<em>柠檬酸rodentium</em在小鼠感染

DOI:

10.3791/50450-v

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12:38 min

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August 13, 2013

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James William Collins, Jeffrey A Meganck, Chaincy Kuo, Kevin P Francis, Gad Frankel

¹MRC Centre for Molecular Bacteriology and Infection, Division of Cell & Molecular Biology,Imperial College London, ²Preclinical Imaging,Caliper- A PerkinElmer Company

Capítulos

00:05Título
01:21Preparing the Bacteria
02:44Infection of Mice with Bioluminescent C. rodentium
04:09Daily Composite 3D Diffuse Light Imaging Tomography with Integrated μCT Imaging (DLIT-μCT) of Infected Mice
06:153D Reconstruction of DLIT-μCT Imaging Data
09:29Generation of a 4D Movie of C. rodentium Infection
10:24Monitored Infection with 5 x 109 cfu C. rodentium, Strain ICC180
12:16Conclusion

Summary

Tadução automática

多模态成像是一种有价值的方法，在小动物模型研究细菌定植。此协议概述感染小鼠生物发光<em>柠檬酸rodentium</em和纵向监测细菌定植复合3D弥漫的光成像断层扫描成像μCT创建一个4D电影<em> C。 rodentium</em>感染。

Tags

Keywords: 4D Multimodality Imaging Citrobacter Rodentium Bioluminescence DLIT-μCT Longitudinal Monitoring Bacterial Infection Colitis Mouse Model IVIS Spectrum CT 3D Optical-CT Co-registration Low-dose μCT Bacterial Colonization Dynamics

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