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Adenovirale transductie van Naïeve CD4 T-cellen aan Treg Differentiatie Studie
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Adenoviral Transduction of Naive CD4 T Cells to Study Treg Differentiation
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Adenovirale transductie van Naïeve CD4 T-cellen aan Treg Differentiatie Studie

DOI:
10.3791/50455-v

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15:33 min

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August 13, 2013

•

,
1Institute for Molecular Immunology,Helmholtz Zentrum München

Capítulos

  • 00:05Título
  • 02:03Cloning of a Gene of Interest into an Entry Vector
  • 03:16Generation of the Primary Virus Lysate
  • 05:47Virus Amplification
  • 07:34T Cell Infection
  • 09:01T Cell Activation and Polarization
  • 10:01T Cell Fixation Staining for Flow Cytometry
  • 11:51Infection Does Not Interfere with T Cell Activation
  • 14:42Conclusion

Summary

Tadução automática

Tadução automática

Adenovirale genoverdracht in naïeve CD4 T-cellen met transgene expressie van de Coxsackie adenovirus receptor kan de moleculaire analyse van regulatoire T celdifferentiatie<em> In vitro</em>.

Tags

Adenoviral TransductionNaive CD4 T CellsRegulatory T CellsTreg DifferentiationEctopic Gene ExpressionCoxsackie Adenovirus ReceptorTCR ActivationTGF-betaIL-2

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