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Tissue Engineering af et menneske 3D<em> In vitro</em> Tumor Test System
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Tissue Engineering of a Human 3D in vitro Tumor Test System

Tissue Engineering af et menneske 3D<em> In vitro</em> Tumor Test System

DOI:
10.3791/50460-v

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11:12 min

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August 06, 2013

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, , , , , ,
1Department of Tissue Engineering and Regenerative Medicine,University Hospital Würzburg

Capítulos

  • 00:05Título
  • 02:16Decellularization of the BioVaSc
  • 03:52Isolation of Primary Human Dermal Microvascular Endothelial Cells (mvECs) and Fibroblasts
  • 05:50Tumor Test System A. Static Culture
  • 07:36Tumor Test System B. Dynamic Culture
  • 08:45Results: Immunohistological Characterization of 3D Tumor Models
  • 10:40Conclusion

Summary

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Tadução automática

Metoder til at skabe menneskelige 3D tumorvæv som testsystemer er beskrevet. Disse teknologier er baseret på et decellulariserede biologiske vaskulariseret Scaffold (BioVaSc), primære humane celler og tumor-cellelinie, som kan dyrkes under statiske såvel som under dynamiske forhold i en strøm bioreaktoren.

Tags

Keywords: 3D Tumor ModelPesquisa do CâncerMalignant Peripheral Nerve Sheath Tumors (MPNSTs)Decellularized Biological ScaffoldBioVaScStatic CultureDynamic BioreactorCell-cell InteractionsIn Vitro Model

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