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Biokemisk Titrering af glykogen<em> In vitro</em
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JoVE Journal Biologia
Biochemical Titration of Glycogen In vitro

Biokemisk Titrering af glykogen<em> In vitro</em

DOI:
10.3791/50465-v

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07:16 min

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November 24, 2013

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, , ,
1Institute for Research on Cancer and Ageing of Nice, UMR CNRS 7284 – INSERM, U1081 – UNS, Centre Antoine Lacassagne,University of Nice – Sophia Antipolis

Capítulos

  • 00:05Título
  • 00:54Cell Lysis
  • 02:50Hydrolysis of Glycogen
  • 03:55Development and Reading of Fluorescence
  • 05:28Results: Glycogen Quantification and Its Role in Hypoxia
  • 06:49Conclusion

Summary

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Vi beskriver her en præcis, reproducerbar og bekvem biokemisk metode til titrering af glykogen in vitro. Denne teknik bruger Abcam glykogen assaykittet og er baseret på successiv hydrolyse af glykogen til glukose og glukose titrering med fluorescens.

Tags

GlycogenBiochemical TitrationIn VitroGlucoseGlucoamylaseOxiRed ProbeFluorescenceCell LineCCL39LS174NormoxiaHypoxia

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