Journal
/
Medicina
/
Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time
JoVE Journal
Medicina
Author Produced
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Medicina
Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time

Transplantation into the Anterior Chamber of the Eye for Longitudinal, Non-invasive In vivo Imaging with Single-cell Resolution in Real-time

DOI:
10.3791/50466-v

•

05:54 min

•

March 10, 2013

•

, ,
1Diabetes Research Institute,University of Miami Miller School of Medicine, 2Department of Surgery,University of Miami Miller School of Medicine, 3Department of Medicine,University of Miami Miller School of Medicine, 4Department of Physiology & Biophysics,University of Miami Miller School of Medicine, 5The Rolf Luft Research Center for Diabetes and Endocrinology,Karolinska Institutet

Capítulos

  • 00:01Título
  • 00:43Motorized-syringe driver with syringe assembled
  • 00:56Loading islets into cannula
  • 03:13Islet Transplantation
  • 05:00Representative Results

Summary

Tadução automática

Tadução automática

A new approach combining intraocular transplantation and confocal microscopy enables longitudinal, non-invasive real-time imaging with single-cell resolution within grafted tissues in vivo. We demonstrate how to transplant pancreatic islets into the anterior chamber of the mouse eye.

Tags

Keywords: Intravital ImagingNon-invasive ImagingLongitudinal ImagingSingle-cell ResolutionAnterior Chamber Of The EyeTransplantationPancreatic IsletIn VivoDrug ScreeningSignal TransductionCancerAutoimmune Disease

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/50466?article_type=v

High-frequency High-resolution Echocardiography: First Evidence on Non-invasive Repeated Measure of Myocardial Strain, Contractility, and Mitral Regurgitation in the Ischemia-reperfused Murine Heart
check_url/pt/50466?article_type=v

Multispectral Real-time Fluorescence Imaging for Intraoperative Detection of the Sentinel Lymph Node in Gynecologic Oncology
check_url/pt/50466?article_type=v

Non-invasive Imaging of Acute Allograft Rejection after Rat Renal Transplantation Using 18F-FDG PET
check_url/pt/50466?article_type=v

Development of an Audio-based Virtual Gaming Environment to Assist with Navigation Skills in the Blind
check_url/pt/50466?article_type=v

Chemotherapy-induced Vascular Toxicity – Real-time In vivo Imaging of Vessel Impairment
check_url/pt/50466?article_type=v

Non-invasive Assessment of the Efficacy of New Therapeutics for Intestinal Pathologies Using Serial Endoscopic Imaging of Live Mice
check_url/pt/50466?article_type=v

Encapsulation Thermogenic Preadipocytes for Transplantation into Adipose Tissue Depots
check_url/pt/50466?article_type=v

Extended Time-lapse Intravital Imaging of Real-time Multicellular Dynamics in the Tumor Microenvironment
check_url/pt/50466?article_type=v

Longitudinal In Vivo Imaging of the Cerebrovasculature: Relevance to CNS Diseases
check_url/pt/50466?article_type=v

Re-Arterialized Rat Partial Liver Transplantation with an in vivo Vessel-Oriented 70% Hepatectomy

Read Article