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JoVE Journal Bioengineering
Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution
 

Measurement of Tension Release During Laser Induced Axon Lesion to Evaluate Axonal Adhesion to the Substrate at Piconewton and Millisecond Resolution

Article DOI: 10.3791/50477-v • 09:31 min • May 27th, 2013
May 27th, 2013 •
1, 2, 3
1Institute of Biophysics, National Research Council of Italy, 2Dipartimento di Sistemi e Informatica, Università di Firenze, 3Department of Neuroscience and Brain Technologies, Istituto Italiano di Tecnologia

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We measured the tension release in an axon that was partially lesioned with a laser dissector by simultaneous force spectroscopy measurement performed on an optically-trapped probe adhered to the membrane of the axon. The developed experimental protocol evaluates the axon adhesion to the culture substrate.

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Tension Release Laser-induced Axon Lesion Axonal Adhesion Substrate Piconewton Resolution Millisecond Resolution Neurite Growth Chemical Signals Mechanical Signals Cell Maturation Scaffolds Cell Adhesion Cytoskeletal Coupling Neuronal Regeneration Force Spectroscopy Measurements Interferometric Tracking Optically Trapped Probe Membrane Of The Axon Piconewton Sensitivity Millisecond Time Resolution Mechanical Coupling Pharmacological Treatment Mechanical Properties Of The Substrate
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