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富亮氨酸的代谢标记重复激酶1和2与放射性磷酸盐
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Biologia
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JoVE Journal Biologia
Metabolic Labeling of Leucine Rich Repeat Kinases 1 and 2 with Radioactive Phosphate
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富亮氨酸的代谢标记重复激酶1和2与放射性磷酸盐

DOI:
10.3791/50523-v

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11:31 min

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September 18, 2013

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, ,
1Laboratory for Neurobiology and Gene Therapy, Department of Neurosciences,KU Leuven and Leuven Institute for Neuroscience and Disease (LIND)

Capítulos

  • 00:05Título
  • 02:02Metabolic Labeling of Cells
  • 05:50Analysis of Protein Labeling
  • 09:46Results: Metabolic Labeling of LRRK1 and LRRK2
  • 10:57Conclusion

Summary

Tadução automática

Tadução automática

富含亮氨酸的重复激酶1和2（LRRK1和LRRK2）是多结构域蛋白，其编码GTP酶都和激酶结构域和它们磷酸化的细胞中。在这里，我们提出了一个协议，以32磷正磷酸盐标记LRRK1和LRRK2的细胞，从而提供衡量其整体的细胞磷酸化水平的一种手段。

Tags

Keywords: Metabolic LabelingLeucine Rich Repeat Kinases 1 And 2 (LRRK1LRRK2)Radioactive PhosphateProtein PhosphorylationImmunoprecipitationSDS-PAGEAutoradiographyWestern Blotting

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