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Capítulos
- 00:05Título
- 01:35Plasma Membrane Purification
- 03:56Detergent Resistant Membrane Preparation
- 05:33Extraction of Proteins from the Detergent Resistant Fraction and In-solution Trypsin Digestion
- 07:04Use of C18-StageTips for Peptide Desalting and Concentration
- 08:48Results: Comparative Proteomic Analysis
- 11:00Conclusion
Here we describe a robust method for the fractionation of plant plasma membranes into detergent resistant and detergent soluble membranes based on a mixture of unlabeled and in vivo fully 15N labeled Arabidopsis thaliana cell cultures. The procedure is applied for comparative proteomic studies to understand signaling processes.
