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Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents
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Neurociência
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JoVE Journal Neurociência
Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents

Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents

DOI:
10.3791/50543-v

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09:40 min

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July 10, 2013

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, , , ,
1Division of Marine Biology and Fisheries, Rosenstiel School of Marine and Atmospheric Sciences,University of Miami

Capítulos

  • 00:05Título
  • 01:44Day One: Cell Preparation
  • 04:12Day Two: Neuron Isolation
  • 08:24Patch Clamp Recordings of Glutamatergic Currents in the Sensory Neurons of Aplysia
  • 08:49Conclusion

Summary

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We describe the dissection of the nervous system of the marine sea hare Aplysia after anesthesia, the isolation of neurons for short term-tissue culture, and recordings of single cell ion currents via the patch clamp technique.

Tags

Aplysia CalifornicaSensory NeuronsPatch ClampGlutamatergic CurrentsMarine Gastropod MolluskNervous System FunctionLearning And MemoryNeuronal Co-cultureDissociated NeuronsIon CurrentsBiophysical CharacterizationPleural GangliaBuccal Ganglia

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