É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:58Subcutaneous Implantation of Tumor Fragments
- 02:54Subcutaneous Injection of Tumor Cells
- 03:23Partial Hepatectomy to Facilitate Intrahepatic Engraftment of Human Tumor Tissue
- 05:37Intrahepatic Implantation of Tumor Fragments
- 06:12Intrahepatic Implantation of Tumor Cells via Direct Injection into the Liver
- 06:52Intrahepatic Xenografting of Tumor Cells via Injection into the Spleen
- 08:01Results: Representative Intrahepatic Xenograft Images
- 10:06Conclusion
Human tumor xenografts in immunodeficient mice are valuable tools to study cancer biology. Specific protocols to generate subcutaneous and intrahepatic xenografts from human hepatocellular carcinoma cells or tumor fragments are described. Liver regeneration induced by partial hepatectomy in recipient mice is presented as a strategy to facilitate intrahepatic engraftment.
