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合成，细胞和交货<em>在体内</em>的树状为基础的pH值传感器应用

JoVE Journal
Química

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JoVE Journal Química

Synthesis, Cellular Delivery and In vivo Application of Dendrimer-based pH Sensors

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合成，细胞和交货<em>在体内</em>的树状为基础的pH值传感器应用

DOI:

10.3791/50545-v

•

16:19 min

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September 10, 2013

•

Lorenzo Albertazzi, Barbara Storti, Marco Brondi, Sebastian Sulis Sato, Gian Michele Ratto, Giovanni Signore, Fabio Beltram

¹Institute for Complex Molecular Systems & Laboratory of Macromolecular and Organic Chemistry,Eindhoven University of Technology & NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, ²NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR & IIT@NEST,Center for Nanotechnology Innovation, ³NEST, Scuola Normale Superiore and Istituto Nanoscienze-CNR, ⁴IIT@NEST,Center for Nanotechnology Innovation

Capítulos

00:05Título
02:30Synthesis of the Sensors
04:20In vitro pH Measurements
05:36Cell Culture and Electroporation
06:38pH Sensing in Living HeLa Cells
08:47In vivo Sample Preparation
10:30pH Imaging in Mouse Brain
12:24Representative Analysis of Dendrimer-based pH Sensor Fluorescence
15:45Conclusion

Summary

Tadução automática

荧光传感器是在生命科学的强大工具。这里我们描述的方法来合成，并使用树枝状聚合物为基础的荧光传感器来测量pH值在活细胞和活体 。树突状支架增强的共轭荧光染料从而提高传感特性的属性。

Tags

Keywords: Dendrimer PH Sensor Fluorescent Indicator Cellular Delivery In Vivo Application Ratiometric Imaging Monodispersity Multivalency Biomedical Device

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