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Forbedring succesraten for proteinkrystallisation af Random Microseed Matrix Screening
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Biologia
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JoVE Journal Biologia
Improving the Success Rate of Protein Crystallization by Random Microseed Matrix Screening

Forbedring succesraten for proteinkrystallisation af Random Microseed Matrix Screening

DOI:
10.3791/50548-v

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12:24 min

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August 31, 2013

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, , , , , ,
1School of Biochemistry,University of Bristol, 2Douglas Instruments

Capítulos

  • 00:05Título
  • 01:58Preparation of the Seed Stock
  • 06:09Establishment of Crystallization Trays
  • 08:45Inspection of Crystallization Trays
  • 09:40Representative rMMS Experiment
  • 11:36Conclusion

Summary

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Her beskriver vi en generel metode til tilfældig microseed matrix screening. Denne teknik er vist signifikant at øge succesraten af ​​protein krystallisering screening eksperimenter, reducere behovet for optimering, og giver en pålidelig forsyning af krystaller til dataindsamling og ligand-iblødsætning eksperimenter.

Tags

Keywords: Protein CrystallizationRandom Microseed Matrix ScreeningRMMSVapor DiffusionSitting DropHanging Drop96-well24-wellLiquid Handling RoboticsProtein Phase DiagramMetastable ZoneCrystal QualityCrystal SupplySoaking Experiments

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