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功能研究和过继转移实验小鼠骨髓嗜中性粒细胞的分离，纯化及贴标

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Imunologia e Infecção

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JoVE Journal Imunologia e Infecção

Isolation, Purification and Labeling of Mouse Bone Marrow Neutrophils for Functional Studies and Adoptive Transfer Experiments

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功能研究和过继转移实验小鼠骨髓嗜中性粒细胞的分离，纯化及贴标

DOI:

10.3791/50586-v

•

07:28 min

•

July 10, 2013

•

Muthulekha Swamydas, Michail S. Lionakis

¹Fungal Pathogenesis Unit, Laboratory of Clinical Infectious Diseases,National Institute of Allergy and Infectious Diseases, NIH

Capítulos

00:05Título
01:40Isolation of Mouse Bone Marrow Cells
04:14Neutrophil Density Gradient Centrifugation Separation and CellTracker Dye-labeling
05:50Results: Bone Marrow-isolated Neutrophils are Highly Purified, Highly Viable, and Trackable by Dye-labeling in Various Mouse Tissues after Adoptive Transfer
07:07Conclusion

Summary

Tadução automática

我们描述了一个协议，用于分离和纯化从小鼠骨髓中的嗜中性粒细胞通过密度梯度离心和嗜中性粒细胞标记使用CellTracker染料。这是一个简单，快速，重复性好，经济的方法获得大量的嗜中性粒细胞的下游功能研究或过继转移和跟踪实验。

Tags

Bone Marrow Neutrophils Isolation Purification Labeling Functional Studies Adoptive Transfer Innate Immunity Inflammation Cell Tracking

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