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Genetisk manipulering i<em> Δku80</em> Stammer for Funksjonell genom analyse av<em> Toxoplasma gondii</em
JoVE Journal
Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Genetic Manipulation in Δku80 Strains for Functional Genomic Analysis of Toxoplasma gondii

Genetisk manipulering i<em> Δku80</em> Stammer for Funksjonell genom analyse av<em> Toxoplasma gondii</em

DOI:
10.3791/50598-v

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09:52 min

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July 12, 2013

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, , , , , ,
1Department of Microbiology and Immunology,The Geisel School of Medicine at Dartmouth

Capítulos

  • 00:05Título
  • 01:58Preparation of Targeting DNA Plasmid p&#29;GOI
  • 02:48Transfecting T. gondii Parasites
  • 03:43Selecting Transfected T. gondii Parasites
  • 07:07Deletion of HXGPRT Marker and C-terminal Tagging
  • 07:48Results: Deletion of rop18 and Removal of the HXGPRT Selectable Marker from gra2
  • 08:54Conclusion

Summary

Tadução automática

Tadução automática

Her rapporterer vi en metode for å bruke type I og type II<em> Δku80</em> Stammer av<em> Toxoplasma gondii</em> Å effektivt generere målrettede genet slettinger og genet erstatninger for funksjonell genomisk analyse.

Tags

Genetic ManipulationΔKU80 StrainsToxoplasma GondiiFunctional Genomic AnalysisHomologous RecombinationGene DeletionGene ReplacementGene TaggingHXGPRT Selectable Marker

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