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立体定向显微注射病毒载体表达Cre重组酶研究的靶基因的作用在可卡因条件性位置偏爱
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Stereotaxic Microinjection of Viral Vectors Expressing Cre Recombinase to Study the Role of Target Genes in Cocaine Conditioned Place Preference
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立体定向显微注射病毒载体表达Cre重组酶研究的靶基因的作用在可卡因条件性位置偏爱

DOI:
10.3791/50600-v

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08:22 min

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July 30, 2013

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1Graduate Program in Neuroscience,Weill Cornell Graduate School of Biomedical Sciences, 2Division of Pediatric Neurology, Department of Pediatrics,Weill Cornell Medical College

Capítulos

  • 00:05Título
  • 01:36Preparation and Setup for Stereotaxic Delivery of Viral Vectors
  • 02:32Stereotaxic Microinjection of Viral Vectors
  • 04:41Conditioned Place Reference: Acquisition, Extinction, and Reinstatement
  • 05:55Representative Cocaine Conditioned Place Preference Results
  • 07:39Conclusion

Summary

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本文介绍如何microinject病毒载体到小鼠大脑中，然后在条件性位置偏爱的范例，包括收购，灭绝和恢复阶段测试。

Tags

Stereotaxic MicroinjectionViral VectorsCre RecombinaseConditional Gene KnockoutCocaineConditioned Place PreferenceAddictionBehavioral ModelsTemporally-specificRegionally-specificGene DeletionGene TargetingRAAVCocaine-induced PlasticityWithdrawalExtinctionReinstatement

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