Journal
/
Biologia
/
The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells
JoVE Journal
Biologia
This content is Free Access.
JoVE Journal Biologia
The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells

The Tomato/GFP-FLP/FRT Method for Live Imaging of Mosaic Adult Drosophila Photoreceptor Cells

DOI:
10.3791/50610-v

•

09:33 min

•

September 20, 2013

•

, , , ,
1Laboratory of Molecular Biology of the Cell,Ecole Normale Supérieure de Lyon, 2INSERM U744, Institut Pasteur de Lille,Université Lille-Nord de France, 3Howard Hughes Medical Institute, Laboratory of Apoptosis and Cancer,The Rockefeller University

Capítulos

  • 00:05Título
  • 02:14Preparing Drosophila for Photoreceptor Visualization
  • 04:29Visualizing the Photoreceptors
  • 06:01Time-course Visualization of Photoreceptor Cells
  • 07:14Results from a High-throughput Screen
  • 09:13Conclusion

Summary

Tadução automática

Tadução automática

The Tomato/GFP-FLP/FRT method involves visualizing mosaic photoreceptor cells in living Drosophila. It can be used to follow individual photoreceptor cell fates in the retina for days or weeks. This method is ideal for studies of retinal degeneration and neurodegenerative diseases or photoreceptor cell development.

Tags

DrosophilaPhotoreceptor CellsEyeClonal AnalysisLive ImagingTomato/GFP-FLP/FRTCornea NeutralizationMosaicNeuronal DegenerationPlanar Cell Polarity

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/50610?article_type=v

Live Imaging of Glial Cell Migration in the Drosophila Eye Imaginal Disc
check_url/pt/50610?article_type=v

The Preparation of Drosophila Embryos for Live-Imaging Using the Hanging Drop Protocol
check_url/pt/50610?article_type=v

Live Imaging of the Zebrafish Embryonic Brain by Confocal Microscopy
check_url/pt/50610?article_type=v

Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations
check_url/pt/50610?article_type=v

Preparation of Developing and Adult Drosophila Brains and Retinae for Live Imaging
check_url/pt/50610?article_type=v

Electron Spin Resonance Micro-imaging of Live Species for Oxygen Mapping
check_url/pt/50610?article_type=v

Preparing Individual Drosophila Egg Chambers for Live Imaging
check_url/pt/50610?article_type=v

Live Imaging of GFP-labeled Proteins in Drosophila Oocytes
check_url/pt/50610?article_type=v

4D Imaging of Protein Aggregation in Live Cells
check_url/pt/50610?article_type=v

Live Imaging of Apoptotic Cell Clearance during Drosophila Embryogenesis

Read Article