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Permeabilization of Adhered Cells Using an Inert Gas Jet
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JoVE Journal Bioengenharia
Permeabilization of Adhered Cells Using an Inert Gas Jet

Permeabilization of Adhered Cells Using an Inert Gas Jet

DOI:
10.3791/50612-v

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08:21 min

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September 04, 2013

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, , , , ,
1Chemical Engineering,McGill University, 2Montreal Heart Institute

Capítulos

  • 00:05Título
  • 01:20Development of LabView Program
  • 02:31Preparation of Equipment
  • 03:08Preparation of the Gas Jet Capillary Nozzle Height
  • 03:46Cell Permeabilization Protocol
  • 06:21Results: Helium Gas Jet Creates Micropores in the Cell Membrane
  • 07:57Conclusion

Summary

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Tadução automática

This protocol describes a method for the temporary permeabilization of adherent cells using an inert gas jet. This technique facilitates the transfer of genetic material and biomolecules into adherent mammalian cells by the utilization of mechanical forces to disrupt the plasma membrane.

Tags

Keywords: Cell PermeabilizationInert Gas JetMechanical TransfectionAdherent CellsIn VitroIn VivoShear ForcesMicroporesHeLaHEK293Human Abdominal Aortic Endothelial CellsGenetic MaterialBiomoleculesCell DamageCell Detachment

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