/
/
Contrôle quantitatif et temporelle des micro-environnement de l'oxygène à l'Islet Plain
É necessária uma assinatura da JoVE para visualizar este conteúdo. Faça login ou comece sua avaliação gratuita.
Capítulos
- 00:05Título
- 01:59Microfluidic Platform Fabrication
- 05:17Microdispenser Setup
- 06:53Isolation and Staining of Mouse Islets
- 08:25Simultaneous Oxygen and Glucose Stimulations
- 09:19Results: Intermittent Hypoxia Preconditioning Improves Islet Responses to Hypoxia
- 10:46Conclusion
Microfluidic oxygen control confers more than just convenience and speed over hypoxic chambers for biological experiments. Especially when implemented via diffusion through a membrane, microfluidic oxygen can provide simultaneous liquid and gas phase modulations at the microscale-level. This technique enables dynamic multi-parametric experiments critical for studying islet pathophysiology.
