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一个微流控芯片单细胞的多功能化学分析
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JoVE Journal Bioengenharia
A Microfluidic Chip for the Versatile Chemical Analysis of Single Cells
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一个微流控芯片单细胞的多功能化学分析

DOI:
10.3791/50618-v

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15:41 min

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October 15, 2013

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, , ,
1Department of Chemistry and Applied Biosciences,ETH Zurich, Switzerland

Capítulos

  • 00:05Título
  • 02:13Fabrication of the Microfluidic Chip
  • 05:31Chip Preparation for Enzymatic Assays
  • 07:15Additional Chip Preparation for Immunoassays
  • 10:05Cell Harvesting and Glucose 6-Phosphate Dehydrogenase Assay
  • 12:08Results: Enzymatic and Antibody Analysis of Single Cells
  • 15:08Conclusion

Summary

Tadução automática

Tadução automática

在这篇文章中，我们提出了一个微流控芯片单细胞分析。它使细胞内的蛋白质，酶，辅因子，以及第二信使通过荧光测定法或免疫测定装置的量化。

Tags

Microfluidic ChipSingle-cell AnalysisIntracellular BiomoleculesQuantitative DeterminationCell IsolationCell LysisLysate RetentionHigh SensitivityArray FormatAnalysis Of ProteinsEnzymesCofactorsSecond Messengers

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