Journal
/
Neurociência
/
A Computer-assisted Multi-electrode Patch-clamp System
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
A Computer-assisted Multi-electrode Patch-clamp System

A Computer-assisted Multi-electrode Patch-clamp System

DOI:
10.3791/50630-v

•

11:01 min

•

October 18, 2013

•

,
1Laboratory of Neural Microcircuitry – Brain Mind Institute,Ecole Polytechnique Federale de Lausanne

Capítulos

  • 00:05Título
  • 01:22Cell Selection
  • 02:23Prepare the Pipettes and Locate the Pipette Tips
  • 04:07Approaching the Cells
  • 05:38Establish the Cell-attached and Whole-cell Configuration for Recording
  • 07:58Results: A Computer-assisted Multi-electrode Patch-clamp System
  • 10:16Conclusion

Summary

Tadução automática

Tadução automática

Multi-electrode patch-clamp recordings constitute a complex task. Here we show how, by automating of many of the experimental steps, it is possible to accelerate the process leading to qualitative improvement in performance and number of recordings.

Tags

Multi-electrode Patch-clampComputer-assisted SystemAutomationExperimental StepsHuman Error ReductionExperiment ControlsWireless GamepadExperiment-related InformationGuidance Cues

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

check_url/pt/50630?article_type=v

Patch Clamp Recordings from Mouse Retinal Neurons in a Dark-adapted Slice Preparation
check_url/pt/50630?article_type=v

Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips
check_url/pt/50630?article_type=v

Patch Clamp Recordings in Inner Ear Hair Cells Isolated from Zebrafish
check_url/pt/50630?article_type=v

F1FO ATPase Vesicle Preparation and Technique for Performing Patch Clamp Recordings of Submitochondrial Vesicle Membranes
check_url/pt/50630?article_type=v

Whole Cell Patch Clamp for Investigating the Mechanisms of Infrared Neural Stimulation
check_url/pt/50630?article_type=v

Isolation of Sensory Neurons of Aplysia californica for Patch Clamp Recordings of Glutamatergic Currents
check_url/pt/50630?article_type=v

Patch Clamp Recordings from Embryonic Zebrafish Mauthner Cells
check_url/pt/50630?article_type=v

Whole-cell Patch-clamp Recordings from Morphologically- and Neurochemically-identified Hippocampal Interneurons
check_url/pt/50630?article_type=v

Whole-cell Patch-clamp Recordings in Brain Slices
check_url/pt/50630?article_type=v

Whole-cell Patch-clamp Recordings for Electrophysiological Determination of Ion Selectivity in Channelrhodopsins

Read Article