Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, <i>Hirudo medicinalis</i>

Josh Titlow; Zana R. Majeed; John G Nicholls; Robin L. Cooper

doi:10.3791/50631

Journal

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Neurociência

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Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis

JoVE Journal
Neurociência

This content is Free Access.

JoVE Journal Neurociência

Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis

Intracellular Recording, Sensory Field Mapping, and Culturing Identified Neurons in the Leech, Hirudo medicinalis

DOI:

10.3791/50631-v

•

11:53 min

•

November 04, 2013

•

Josh Titlow, Zana R. Majeed², John G Nicholls, Robin L. Cooper

¹Department of Biology,University of Kentucky, ²Department of Biology, College of Science,University of Salahaddin, Iraq, ³Department of Neurobiology and Cognitive Neuroscience,SISSA, Italy

Capítulos

00:05Título
01:30An Introduction from John Nicholls
03:59Ventral Ganglion Dissection
05:36Culturing Neurons from the Ventral Ganglion Preparation
07:26Ganglion-body Wall Preparation
08:46Interpreting Recordings and Making Dye Injections
11:07Conclusion

Summary

Tadução automática

This article describes three nervous system preparations using leeches: intracellular recording from neurons in ventral ganglia, culturing neurons from ventral ganglia, and recording from a patch of innervated skin to map sensory fields.

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