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成比例的生物传感器测量活酵母细胞中的线粒体氧化还原状态和ATP

JoVE Journal
Bioengenharia

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JoVE Journal Bioengenharia

Ratiometric Biosensors that Measure Mitochondrial Redox State and ATP in Living Yeast Cells

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成比例的生物传感器测量活酵母细胞中的线粒体氧化还原状态和ATP

DOI:

10.3791/50633-v

•

12:22 min

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July 22, 2013

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Jason D. Vevea*¹, Dana M. Alessi Wolken*¹, Theresa C. Swayne, Adam B. White, Liza A. Pon

¹Department of Pathology and Cell Biology,Columbia University, ²Herbert Irving Comprehensive Cancer Center,Columbia University

Capítulos

00:05Título
02:48Growth of Cells and Preparation for Imaging
03:29Mito-roGFP1 Imaging
04:33MitGO-ATeam2 Imaging
06:09ImageJ Analysis
08:40Measuring Mitochondrial Redox State and ATP with Mito-roGFP1 and MitGO-ATeam2
11:22Conclusion

Summary

Tadução automática

我们描述了使用两个比例，基因编码生物传感器的，这是基于GFP，监测线粒体的氧化还原状态和细胞内ATP水平分辨率在活酵母细胞。

Tags

Keywords: Mitochondrial Redox State Mitochondrial ATP Ratiometric Biosensors RoGFP MitGO-ATeam FRET Yeast Cells Energy Metabolism Calcium Homeostasis Cellular Lifespan Programmed Cell Death

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