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无标记的分离和细胞的富集通过非接触式介电泳
JoVE Journal
Medicina
This content is Free Access.
JoVE Journal Medicina
Label-free Isolation and Enrichment of Cells Through Contactless Dielectrophoresis
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无标记的分离和细胞的富集通过非接触式介电泳

DOI:
10.3791/50634-v

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10:38 min

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September 03, 2013

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, , ,
1School of Biomedical Engineering and Science,Virginia Tech, 2Department of Engineering Science and Mechanics,Virginia Tech

Capítulos

  • 00:05Título
  • 01:54Fabricating a Prototype cDEP Microfluidic Device
  • 03:12Preparing a Cell Suspension in DEP Buffer
  • 03:57Loading the cDEP Microfluidic Chip
  • 06:04Characterizing Crossover Frequency of Cells Using cDEP Chip
  • 07:49Varying the Experiment to Perform Cell Sorting or Enrichment
  • 08:43Results: Cell Response and Sorting by Contactless Dielectrophoresis
  • 09:50Conclusion

Summary

Tadução automática

Tadução automática

非接触式介电电泳（CDEP）达到通过其固有的介电性能和分拣颗粒的富集。流体通道的电极取代金属电极的传统，以DEP，适合CDEP非破坏无菌表征和生物粒子进行排序。我们将演示如何编写一个CDEP微型设备，并进行细胞鉴定和分选实验。

Tags

Keywords: DielectrophoresisContactless DielectrophoresisCDEPLabel-free Cell IsolationCell EnrichmentMicroparticle SortingSoft LithographyCrossover FrequencyOvarian Cancer CellsFluorescing Microspheres

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